The charity bike riders from HMS Ledbury are due to arrive in town around noon on Thursday, February 12.
Mayor Keith Francis is appealing for people to turn out and give the fundraisers a warm welcome.
He said: "It will be nice to have a lot of people out and about in the street to greet them."
The sailors will have pedalled all the way from Plymouth and Coun Francis has been in training so he can join them on the last leg from Gloucester.
The crew are tackling the ride to raise funds for Marie Curie Cancer Care and to further cement links between the Royal Navy mine sweeper and the town.
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